BMBF project (13N13698): LegioTyper

Culture-independent serotyping of Legionella pneumophila in water, aerosol and urine samples

A rapid bioanalytical method for the detection of Legionella pneumophila will be established. The sandwich microarray immunoassay will detect serogroup 1 to 15 by monoclonal serotyping on the microarray analysis platform MCR 3 in not more than one hour. Patterns of specific microarray signals will be compared by the analysis of environmental (water and bioaerosols) and clinical samples (e.g. urine). Matching signal patterns will give the direct link between an infection and the source that is responsible for a legionellosis outbreak.

Cooperation partners: Technische Universität Dresden, Dresden (TUD); Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Erlangen (LGL); GWK Präzisionstechnik GmbH, München

Funding: Bundesministerium für Bildung und Forschung (BMBF)

Homepage: www.legiotyper.de

IGSSE Project (9.06): MiCSMaP

Microreactor with integrated characterization for the synthesis of magnetic nanoparticles.

MiCSMap is an interdisciplinary project founded by the International Graduate School of Science and Engineering (IGSSE) between analytical chemistry, chemical engineering and electrical engineering. Research interests are the synthesis and coating of magnetic nanoparticles in microfluidic reactors with integrated continuous magnetic separation and particle characterization.

Cooperation partner: Dr.-Ing. Bernhard Gleich (IMETUM, School of Bioengineering, Technische Universität München)

JPI Water Project (WU1346A): METAWATER

New metagenomics and molecular based tools for European scale identification and control of emergent microbial contaminants in irrigation water (METAWATER).

European project analyzing fecal contaminations, emergent and ESBL-producing pathogens in irrigation water by monolithic adsorption filtration (MAF) combined with qPCR and multiplex DNA microarray analysis together with next generation sequencing.

Cooperation partner: Dr. Anna Charlotte Schultz (DTU, Copenhagen, Denmark), Dr. José Luis Alonso (UPV, Valencia, Spain), Prof. Rosina Girones (UB, Barcelona, Spain), Prof. Maria José Figueras (URV, Tarragona, Spain), Dr. Georgios T. Papageorgiou (State General Laboratory, Cyprus) and Prof. Dr. Christiane Höller (LGL, Oberschleißheim, Germany)

Funding: Bundesministerium für Bildung und Forschung (BMBF)

Development and validation of an immunological screening method for the determination of toxicologically relevant pyrrolizidine alkaloids in herbal tea and related matrices

Direct or indirect contamination of human food sources like wheat, milk, eggs, honey, salad, herbals, tea and food supplements with pyrrolizidine alkaloids (PA) are, depending on PA concentration and duration of its intake, supposed to give rise to acute (liver failure) or chronic (pulmonary arterial hypertension, cancer and teratogenic effects) toxicity. Food industries are interested in an in-house method for easy and effective analysis of unprocessed plant resources etc. Bioanalytical methods based on immunological detection of PA are most suitable in this context, as it provides parallel processing of many samples and requires minor trained staffing. Moreover, it is cheap and does not require any costly sample preparation. In order to address food industries demands, the aim of this project is to develop and validate an immunological screening method for the determination of toxicologically relevant PAs in herbal tea and related matrices.

Responsible persons: Prof. Dietmar Knopp and Katharina Stutzer, MSc.

Cooperation partners: Prof. Gareis and Dr. Gottschalk (Veterinary Faculty, Chair of Food Safety, Ludwig-Maximilians-University, Oberschleißheim, Germany)

Funding: FEI – Research Association of the German Food Industry (AiF 19010N)

Finalized Projects

BMBF Project (13N12613): LEVERA (15.4.2013-14.07.2016)

Food provision and analytics - Rapid concentration and multiplex microarray analysis of microorganisms and toxins

The goal of this project was to establish a rapid concentration method in food samples which was compatible to antibody microarrays on the MCR 3 system to detect in parallel and fully automated pathogenic bacteria, leading microorganisms and toxins.

BMBF Project (033W010E): INIS-EDIT (1.6.2013-31.12.2016)

Establishment of a concentration- and detection system for the inline-monitoring of waterborne pathogens in drinking and raw water (EDIT).

It was an interdisciplinary project between analytical chemistry, environmental microbiologists, water scientists, microsystem engineers and companies from the field of diagnostics and water supply to establish a monitoring system for rapidly analyse the water hygiene in water pipes.